By Awtar Krishan,H. Krishnamurthy,Satish Totey

A much-needed primer at the use of laser circulate cytometry for stem telephone analysis

Laser circulate cytometry is a robust device for swift research of cells for marker expression, mobile cycle place, proliferation, and apoptosis. notwithstanding, no assets particularly tackle using this system for the research of stem cells; this is often particularly very important as stem mobile research consists of really expert equipment and marking approaches according to particular features similar to marker expression, mobilephone measurement, drug shipping, and efflux of the stem cells.

Now, this publication studies those approaches, discusses the technological know-how at the back of them, and gives real-world examples to demonstrate the usefulness of the tools. It brings jointly world-class specialists in pathology, biophysics, immunology, and stem phone study, who draw upon their vast adventure with the equipment and convey examples of excellent info to aid advisor researchers within the correct direction.

Chapter insurance includes:

  • Stem cellphone research and sorting utilizing part population
  • Flow cytometry within the research of proliferation and apoptosis
  • Stem cellphone biology and application
  • Identification and isolation of very small embryonic-like stem cells from murine and human specimens
  • Hematopoietic stem cells—issues in enumeration
  • Human embryonic stem cells: long term tradition and cardiovascular differentiation
  • Limbal stem cells and corneal regeneration
  • Flow cytometric sorting of spermatogonial stem cells
  • Breast melanoma stem cells
  • Stem telephone marker expression in cells from physique hollow space fluids

This publication is a vital source for all graduate scholars, practitioners in constructing international locations, libraries and ebook repositories of universities and examine associations, and person researchers. it's also of curiosity to laboratories engaged in stem telephone learn and use of stem cells for tissue regeneration, and to any association dealing in stem telephone and tissue regeneration research.

Show description

Read or Download Applications of Flow Cytometry in Stem Cell Research and Tissue Regeneration PDF

Best cell biology books

Cell Biology of Plant Nematode Parasitism: 15 (Plant Cell Monographs)

Plant-parasitic nematodes are one of the such a lot harmful plant pathogens, inflicting huge, immense losses to agronomic vegetation world wide. This publication offers an up to date evaluate of study concerning of an important nematode pests, root-knot and cyst nematodes. Chapters conceal early plant-nematode interactions, id of nematode proteins very important within the institution of nematode feeding websites, and category of biochemical and signaling pathways major within the improvement of specialised feeding websites within the host.

Constitutive Activity in Receptors and Other Proteins, Part B: 485 (Methods in Enzymology)

This quantity of tools in Enzymology covers the present method for the detection and review of constitutively lively proteins. The chapters written through professional authors who're leaders within the box, supply tricks and tips now not to be had in fundamental examine guides. it really is widely referenced, with valuable figures and tables during the quantity.

Studies on Retinal and Choroidal Disorders (Oxidative Stress in Applied Basic Research and Clinical Practice)

Stories on Retinal and Choroidal issues examines the position that oxidative and nitrosative tension performs within the advanced body structure and pathophysiology of the retina and choroid.  either the fundamental technology researcher and the medical practitioner can use this publication as a advisor to the present figuring out of retinal and choroidal mobile mechanisms curious about getting older and disorder.

Proteases in Apoptosis: Pathways, Protocols and Translational Advances

This booklet offers a entire evaluation of the proteases excited by programmed mobilephone loss of life. It offers a concentrated but huge dialogue on proteolytic enzymes equivalent to caspases, HtrAs, granzymes, calpains and cathepsins in addition to laboratory protocols on the topic of enzymology and apoptosis. Mouse version structures and non-invasive imaging suggestions in apoptosis-related ailments comparable to melanoma and neurodegeneration also are lined during this ebook.

Extra info for Applications of Flow Cytometry in Stem Cell Research and Tissue Regeneration

Example text

Download PDF sample

Rated 4.06 of 5 – based on 30 votes