By Hans-Joachim Müller,Daniel Ruben Prange
Ziel dieses handlichen, deutschsprachigen Laborhandbuches ist es, die regular- und Spezialanwendungen der PCR in praxisnaher und verständlicher shape darzustellen. Es wendet sich an Diplomanden, Doktoranden, Wissenschaftler und TAs, die die Möglichkeiten der PCR für ihre molekularbiologischen oder diagnostischen Fragestellungen nutzen möchten.
In vorliegender 2. Auflage wurden die etablierten Methoden aktualisiert und wichtige neue Applikationen (z.B. subsequent iteration Sequencing oder die Emulsions-PCR) hinzugefügt. Da die Automation bei den molekularbiologischen Applikationen fortschreitet, erfordern gerade die letztgenannten Methoden immer weniger Handarbeit, wobei das generelle Verständnis über die einzelnen Schritte vorhanden sein muss. Dieses ‚Know-How‘ erhalten Sie im vorliegenden PCR-Methodenbuch.
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